The fully automated exosome extraction and concentration instrument extracts and concentrates exosomes from various liquids, including urine, saliva, cerebrospinal fluid, blood, tears, cell/bacterial culture medium, milk, cell-membrane extrusion vesicles, ginseng infusion, and others. It fully automatically extracts exosomes in different size-fraction ranges, producing highly concentrated exosomes rapidly and without contamination while greatly reducing operator workload.

Using multistage pressure-stabilized filtration with pulsatile tangential flow, the instrument fully automates exosome extraction, concentration, purification, and buffer exchange. It is widely used in scientific research on exosomes from cell supernatants, animal body fluids, and plant extracts;

1. Main functional parameters:

(1) Sample-processing volume: two sample-processing modes, covering 10 μL–500 mL and 10,000 mL. Different filter-unit models enable processing of different sample extracts;

(2) Supported sample types: various body fluids, stem-cell supernatants, and raw plant extracts;

(3) Sample-position tube types: reservoir capacities from 15 mL to 450 mL;

(4) Sample-loading method: fully automatic, including automatic loading, concentration, purification, buffer exchange, and collection throughout the entire workflow;

(5) Reagent management: the product includes injectable-grade normal saline and sterile PBS for exosome washing and real-time buffer exchange;

(6) Consumable types: exosome filter units are available in several submodels, as shown in the table below;

(7) Maximum single loading volume: two sample-volume modes are available—a 10–500 mL mode and a 10 L mode—selected according to the filter unit;

(8) Exosome purification and enrichment process: the product provides two-stage purification and concentration. The combination of three functions enables simultaneous exosome purification and concentration;

(9) Purification time: less than 10 minutes for a 200 mL sample;

(10) Processing speed: extraction rate of 1–3 hours per 10 L;

(11) Exosome recovery volume: the volume of exosome concentrate can be set;

(12) Extracellular-vesicle subtype separation: four membrane cassettes with different pore sizes—0.45 μm, 0.22 μm, 0.1 μm, and 0.04 μm—are available. The four different-pore-size membrane cassettes supplied by default filter exosomes that are stored respectively in the primary filter reservoir (particle size 200–450 nm), secondary filter reservoir (particle size 100–200 nm), and tertiary filter reservoir (particle size 30–100 nm). Configurations can be selected according to user requirements.

(13) Temperature control: the product is extensively equipped with 7 weighing sensors, 5 pressure sensors, and 2 temperature sensors. A fuzzy PID control strategy intelligently identifies filter units, automatically concentrates and purifies different exosome volumes, and controls temperature throughout the process;

(14) Transparent viewing door: equipped with multiple weight sensors and using a fuzzy PID control strategy, the instrument intelligently identifies filter units, makes the entire process visible, and automatically concentrates and purifies different exosome volumes;

(15) Purification workflow: the product has multiple sensors, and its control software provides multiple operating modes. Their combinations support dozens of concentration and purification modes, enabling optimization of exosome concentration and purification;

(16) Review-list data storage: equipped with a miniature built-in industrial computer, with 300 GB of historical-data storage sufficient for 10 years;

(17) Screen display: the 11.4-inch display supports barcode scanning and code entry to set initial sample parameters. It displays and stores historical data in real time and provides parameter alarms. Each batch has a fixed code for convenient querying and recordkeeping;

(18) Contamination prevention and control: equipped with two ozone-free UV lamps. Sterilization duration can be set freely, with a default of 30 minutes;

(19) Care and maintenance: supplied with injectable-grade normal saline and sterile PBS. The software includes cleaning workflows for leak detection, filter-unit cleaning, automatic recovery, and other functions;

(20) Contaminating-protein removal rate: >99%;

(21) Exosome recovery rate: >90%;

(22) Loading volume for different sample types: the source text is incomplete after “10-”; the product's functions and extraction categories cover sample loading volumes and types and can meet the requirements;

(23) User interface: Chinese and English;

(24) Weight: ≤120 kg;

(25) Interfaces: 5 USB ports, 1 Ethernet port, 1 serial port, and 1 HD display port;

(26) Recovered concentration: 1.0E+11–13 particles/mL

(27) Filtration shear rate: <2000 s⁻¹, preserving exosome-vesicle integrity;

(28) Consumable packaging: irradiation-sterilized packaging;

(29) Sterile collection: terminal sterile-collection program for improved yield;

(30) Concentration factor: 10–200×;

(31) Purification: simultaneous real-time purification with real-time removal of contaminating proteins and free dyes;

(32) Filter-unit residue: no exosome residue after filtration;

(33) Cloud app/mini program: remotely displays the instrument's operating environment and provides alarm notifications;

(34) Liquid pumping: disposable syringe for low operating costs;

(35) Concentration-factor setting: initial concentrate-volume setting with real-time control;

(36) Concentration: independent two-stage concentration facilitates process development;

(37) Filtration: dynamically rotating filter unit maintains stable internal pressure, extends filter-unit service life, and improves filtration efficiency;

(38) Cleaning: circulating cleaning with alkaline solution and PBS;

(39) Ultrasonic vibration: an ultrasonic-vibration mode optimizes exosome filtration and recovery efficiency;

(40) Processing method: independent, synchronous operation

(41) Clean-air efficiency: ≥99.99% at 0.3 μm; pressure drop ≤130 Pa;

Quality assurance:

Our company undertakes to provide a 5-year warranty for the complete instrument, including wear parts and consumables, plus lifetime maintenance. The free-warranty period begins on the date of acceptance. A stamped, written warranty undertaking from the original manufacturer must be submitted when the contract is signed.