A fully automated multistage-filtration exosome separation instrument was developed and its performance evaluated by extracting exosomes derived from human autologous adipose-derived stem cells. Methods: define the instrument's separation process, design its structure, and complete hardware and software development. Obtain autologous adipose tissue from patients, digest it to isolate adipose-derived stem cells, culture and passage them, collect and preliminarily centrifuge the supernatant from third-passage cells, add the supernatant to the first-stage reservoir, filter it through the instrument, and centrifuge the resulting third-stage filtrate to extract exosomes. Observe exosome morphology by transmission electron microscopy (TransmissionElectronMicroscope, TEM) and perform nanoparticle tracking analysis (NanoparticleT...