Detailed operating steps for a fully automated exosome extraction and concentration instrument

　　Fully Automated Exosome Isolation and Concentration Systemis advanced biological laboratory equipment used primarily for efficient, automated extraction and concentration of exosomes from biological samples. It uses technologies including, but not limited to, pulsed tangential-flow multistage recirculating filtration, intelligent control, and magnetic-bead capture. Pulsed tangential-flow constant-pressure multistage recirculating filtration prevents particles from clogging the membrane and continuously processes large sample volumes at high speed, while magnetic-bead capture specifically binds exosomes for efficient separation and extraction.

　　Fully Automated Exosome Isolation and Concentration Systemoperating steps:

　　1. Sample pretreatment

　　Remove cells and cell debris by centrifugation or filtration: before exosome extraction, pretreat the sample to remove cells and debris, generally by centrifugation or filtration.

　　Separate and purify with EXODUS: place the pretreated sample in the EXODUS fully automated exosome extraction system. Add the liquid to be extracted to the reservoir, then start the equipment for continuous uninterrupted processing.

　　2. Separation and purification

　　A new exosome separation method based on ultrasonic nanofiltration: EXODUS T-2800 uses a negative-pressure oscillation system (NPO) combined with a double-coupled harmonic oscillation system (HO) acting on a nano-ultrafiltration chip to enrich and purify exosomes.

　　3. Collect purified exosomes

　　Use in downstream applications: collect the purified exosomes for subsequent research and applications such as genomic and proteomic analysis, biomolecular drug-delivery studies, and in vitro and in vivo functional analysis.

　　Overall, the fully automated exosome extraction and concentration instrument combines a sophisticated structure with comprehensive functions, meeting exosome extraction and purification needs in different fields. As technology advances and application demand grows, its structure will continue to be optimized and*.